مقاله Differential Detection of ? or ?درGlobin Gene Deletion by
نوشته شده به وسیله ی ali در تاریخ 95/8/29:: 10:40 عصر

مقاله Differential Detection of ? or ?درGlobin Gene Deletion by SemiدرQuantitative Triplex PCR تحت word دارای 3 صفحه می باشد و دارای تنظیمات در microsoft word می باشد و آماده پرینت یا چاپ است
فایل ورد مقاله Differential Detection of ? or ?درGlobin Gene Deletion by SemiدرQuantitative Triplex PCR تحت word کاملا فرمت بندی و تنظیم شده در استاندارد دانشگاه و مراکز دولتی می باشد.
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توجه : در صورت مشاهده بهم ریختگی احتمالی در متون زیر ،دلیل ان کپی کردن این مطالب از داخل فایل ورد می باشد و در فایل اصلی مقاله Differential Detection of ? or ?درGlobin Gene Deletion by SemiدرQuantitative Triplex PCR تحت word ،به هیچ وجه بهم ریختگی وجود ندارد
بخشی از متن مقاله Differential Detection of ? or ?درGlobin Gene Deletion by SemiدرQuantitative Triplex PCR تحت word :
سال انتشار: 1384
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: 3
چکیده:
Prevention of -thal is an ongoing program in Iran. There are a large number of couples who have been suspicious in or -thal. Exact determination being carrier of -thal or -thal at the molecular level is crucial for counseling and prenatal diagnosis. In order to determine the presence or absence of deletion in - , 1- , or 2- globin genes, a triplex PCR is designed which quantitatively determines presence or absence of deletion in either of the above genes. PCR was optimized testing different conditions. Crucial parameters were found to be DNA extraction method (salting out was best), dNTP concentration, and presence of DMSO and glycerol. The study have been done on 10 normal and 50 individuals referred to us as being possible carrier of or . PCR products with intact (no deletion) gene showed band densities different from persons either having -gene deleted or either or both -genes deleted. If no deletion is detected, the sample can be a subject for point mutation study. Several repeated tests with samples known to have deletion in - or -globin genes proved our quantitative method being very accurate and useful.

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